primary adult hpfs 3310  (ScienCell)

Journal: Clinical Science (London, England : 1979)

Article Title: LTBP2 is secreted from lung myofibroblasts and is a potential biomarker for idiopathic pulmonary fibrosis

doi: 10.1042/CS20180435

Figure Lengend Snippet: ( A ) Representative images of FACS analysis for steady-state fibroblasts (FB) from untreated mice (UM) and activated FB/myofibroblasts (MFB) from bleomycin-treated mice (BM). ( B ) Fluorescence intensities of FITC-conjugated anti-αSMA antibody in steady-state FB, activated FB, and MFB ( n =4 per group). Statistical comparisons by t test were performed using the value of steady-state FB as a control. * P <0.05. ( C ) Heatmap generated by microarray analyses. Genes up-regulated in MFB were selected according to the following criteria: (i) five-fold greater than in steady-state FB; (ii) five-fold greater than in total lung homogenates; and (iii) two-fold greater than the mean value of all genes in MFB. Conversely, genes up-regulated in steady-state FB were defined according to the following criteria: (i) five-fold greater than in MFB; (ii) five-fold greater than in total lung homogenates; and (iii) two-fold greater than the mean value of all genes in steady-state FB. The listed genes are the top 20 genes with high signals in each group and are available in the NCBI Reference Sequence.

Article Snippet: Primary human adult lung fibroblasts (HPF-a #3310, passage number: less than three) were purchased from the ScienCell Research Laboratories (U.S.A.).

Techniques: Fluorescence, Control, Generated, Microarray, Sequencing

Journal: Clinical Science (London, England : 1979)

Article Title: LTBP2 is secreted from lung myofibroblasts and is a potential biomarker for idiopathic pulmonary fibrosis

doi: 10.1042/CS20180435

Figure Lengend Snippet: ( A , B ) Ltbp2 and Acta2 mRNA levels in mouse lung steady-state fibroblasts (FB), activated FB, and myofibroblasts (MFB) ( n =3 per group). Statistical comparisons by t test were performed using the value of steady-state FB as a control. * P <0.05, ** P <0.01, *** P <0.001. ( C ) Ltbp2 mRNA levels in lineage cells from untreated and bleomycin-treated mouse lungs ( n =3 per group). There were no statistically significant differences between the two mouse groups ( t test).

Article Snippet: Primary human adult lung fibroblasts (HPF-a #3310, passage number: less than three) were purchased from the ScienCell Research Laboratories (U.S.A.).

Techniques: Control

Journal: Clinical Science (London, England : 1979)

Article Title: LTBP2 is secreted from lung myofibroblasts and is a potential biomarker for idiopathic pulmonary fibrosis

doi: 10.1042/CS20180435

Figure Lengend Snippet: ( A , B ) LTBP2 and Acta2 mRNA levels in human lung fibroblasts with or without 48-h external administration of TGF-β1 ( n =3 per group). Statistical comparisons by t test were performed using the value of the untreated group as a control. * P <0.05, ** P <0.01, *** P <0.001. ( C ) Protein concentrations, measured by ELISA, of LTBP2 in culture supernatant with or without 48-h external administration of TGF-β1 ( n =3 per group). Statistical comparisons by t test were performed using the value of the untreated group as a control. * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: Primary human adult lung fibroblasts (HPF-a #3310, passage number: less than three) were purchased from the ScienCell Research Laboratories (U.S.A.).

Techniques: Control, Enzyme-linked Immunosorbent Assay

Journal: Clinical Science (London, England : 1979)

Article Title: LTBP2 is secreted from lung myofibroblasts and is a potential biomarker for idiopathic pulmonary fibrosis

doi: 10.1042/CS20180435

Figure Lengend Snippet: Genes that are highly upregulated in myofibroblasts (MFB) or steady-state fibroblasts (FB) from mouse lungs

Article Snippet: Primary human adult lung fibroblasts (HPF-a #3310, passage number: less than three) were purchased from the ScienCell Research Laboratories (U.S.A.).

Techniques: